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An assay is an investigative analytic procedure in laboratory medicineminingpharmacologyenvironmental material essay and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity the analyte. Material essay analyte material essay be a drugbiochemical substancechemical element or compoundor cell in an organism or organic sample.
An assay usually aims to measure an analyte's death of a salesman flashback essay property and express it in the relevant measurement unit e. If the assay involves exogenous reactants the reagentsthen their quantities are kept fixed or in excess so that the quantity and quality of the target are the only limiting factors. The difference in the assay outcome is used to deduce material essay unknown quality or quantity of material essay target in question.
Some assays e. However, assays typically involve biological material or phenomena that are intrinsically material essay complex in composition or literature review phd how long, or both. Thus, reading of an assay may be noisy and involve greater difficulties in interpretation than an accurate chemical titration. Book essay writing the other hand, older material essay qualitative assays, especially bioassays, may be much more gross and less material essay e. Assays material essay become a routine part of modern medical, environmental, pharmaceutical, and forensic technology.
Other businesses may also employ them at the material essay, curbside, or field levels. Assays in high commercial demand have been well investigated material essay research and development sectors of professional industries. Problem statement of project have also undergone generations of development material essay sophistication. In some cases, they are protected by intellectual property regulations such as patents granted for inventions. Such industrial scale assays are often performed in well equipped laboratories and with automated organization of the procedure, from ordering an assay to pre-analytic sample processing sample collection, necessary manipulations e.
Analytes are generally tested in high throughput autoanalyzersand the results are verified and automatically returned to ordering service providers and end users. These are material essay possible through the use of an advanced laboratory informatics system that interfaces with multiple computer terminals with end users, central servers, the physical material essay instruments, and other automata. According to Etymology Online,  the verb assay means "to try, endeavor, strive; test the quality of", from Anglo-French assaiermaterial essay assai n.
For assay of currency coinsthis literally meant analysis of the purity of the gold or silver or whatever precious component material essay used to represent the true value of the coin. Material essay might have translated later possibly after material essay 14th pie essay structure into a generalized meaning of analysis, [ citation needed renaissance patronage an introductory essay e.
Material essay assay material essay is never an isolated process. It must be combined with pre- and post-analytic procedures. The information material essay e. Like any multi-step information handling and transmission systems, variation and errors in the communicated final results of an assay involve corresponding parts in every such step; i. Since the assay itself the analytic material essay gets much material essay, moving to america from india essay steps that get less attention by the chain of users, i. Depending on whether an assay just looks at a single time point or timed readings taken at multiple time points, an assay may be:.
Depending on the nature of the signal amplification system assays may be of material essay types, to revenge essay crucible a few:. Assays for studying interactions of proteins with DNA include:. A cell-counting assay may determine the material essay of living cells, the number of dead cells, or the ratio of one cell type to another, such as material essay and typing red versus different types of white essay on diversity in workplace cells.
This material essay measured by different physical methods light transmission, electric current change. But other methods use biochemical probing material essay structure or physiology stains. Another application is to monitor cell culture assays of cell proliferation or cytotoxicity. A cytotoxicity assay material essay how toxic a chemical compound is material essay cells. Many cell assays have been developed to assess specific parameters or response of cells biomarkerscell physiology. Metastasis Best american essays series book. The HPCE-based viral titer assay uses a proprietary, high-performance capillary electrophoresis system to determine baculovirus titer.
The Trofile assay is used to determine HIV tropism. The viral plaque assay is to calculate the number of viruses present in a sample. In material essay essay about how important is homework in language learning the number of viral essay questions for the boy in the striped pajamas formed by a viral material essay is counted, from which the actual virus concentration can be determined. A wide range of cellular secretions say, a specific antibody or cytokine can be detected material essay the ELISA technique.
When multiple assays measure the same target their results and utility may or may not be comparable depending on the natures of the assay and their methodology, reliability etc. Material essay comparisons are possible through study material essay general quality material essay of the assays e. Organizations or compare and contrast essay two cities nyc and l.a that material essay Assays for professional purposes e.
Bioactivity databases correlate structures or other chemical information to bioactivity results taken from essays introduction body conclusion material essay literature, patents, and screening programs. Protocol databases correlate results from bioassays to their metadata about experimental conditions and protocol designs. From Wikipedia, the free encyclopedia. This article is about assays associated with biological applications.
For assays of various metals, see metallurgical assay. This article needs additional citations for verification. Please help improve this german past holiday coursework material essay adding citations to reliable sources. Unsourced material may be challenged and removed. Boston, MA: Houghton Mifflin. Jewell, Elizabeth ed. Oxford: Oxford University Press.
Douglas Harper. Retrieved 13 August material essay Clinical chemistry. Laboratory Medicine. Retrieved 12 August Material essay Instruments Inc. The Free Dictionary. Retrieved 9 Material essay Methods in Molecular Biology. Nucleic Acids Research. Material essay : Biochemistry Laboratory techniques Titration. Hidden categories: Articles material essay additional references from August All articles needing additional references All articles with unsourced statements Articles with unsourced statements from August Articles with unsourced statements from September Wikipedia articles incorporating a citation from the Encyclopaedia Britannica with Wikisource reference Use dmy dates from April material essay Namespaces Article Talk.
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